Polymer-based, Lighter than or Light as Water Scaffolds for Tissue Engineering and Methods for Preparation and Use Thereof

Becker et al., "Three-Dimensional Growth and Differentiation of Ovarian Tumor Cell Line in High Aspect Rotating-Wall Vessel: Morphologic and Embryologic Considerations", J. Cellular Biochem. 1993 51(3):283-289. Burwell R.G. Bone Grafts, Derivatives and Substitutes., M.R. Urist and R.G. Burwell, Editors 1994, Butterworth-Heinemann Ltd.: Oxford. Casser-Bette et al., "Bone Formation by Osteoblast-Like Cells in a Three-Dimensional Cell Culture", Calcified Tissue International 1990 46:46-56. Cook et al., "The Effect of Recombinant Human Osteogenic Protein-1 on Healing of Large Segmental Bone Defects" J. Bone Joint Surg. Am. 1994 76(6):827-838. Devin et al., "Three-dimensional degradable porous polymer-ceramic matrices for use in bone repair", J. Biomater. Science-Polymer Edition 1996 7(8):661-669. Ducheyne et al., "Effect of Bioactive Glass Templates on Osteoblast Proliferation and In Vitro Synthesis of Bone-Like Tissue", J. Cell. Biochem. 1994 56:162-167. El-Ghannam et al., `Bioactive material template for in vitro synthesis of bone" J. Biomed. Mater. Res. 1995 29:359-370. Gadzag et al., "Alternatives to Autogenous Bone Graft: Efficacy and Indications", J. Amer. Acad. Ortho. Surg. 1995 3(1):1-8. Goldstein et al., "Effect of Osteoblastic Culture Conditions on the Structure of Poly(DL-Lactic -co -Glycolic Acid) Foam Scaffolds", Tissue Engineering 1999 5(5):421-433. Granet et al., "Rotating-wall vessels, promising bioreactors for osteoblastic cell culture: comparison with other 3D conditions", Cell Eng. 1998 3:513-519. Ishaug et al., "Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds", J. Biomed. Mater. Res. 1997 36:17-28. Ishaug-Riley et al., Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers, Biomaterials 1998 19:1405-1412. Klement and Spooler, "Utilization of Microgravity Bioreactors for Differentiation of Mammalian Skeletal Tissue", J. Cellular Biochem. 1993 51:252-256. Labarca and Paigen, "A Simple, Rapid, and Sensitive DNA Assay Procedure", Anal. Biochem. 1980 102:344-352. Langer and Vacanti, "Tissue Engineering", Science 1993 260(5110):920-926. Laurencin et al., "Tissue Engineered Bone-Regeneration Using Degradable Polymers:The Formation of Mineralized Matrices", Bone 1996 19(1):93S-99S. Laurencin et al., "A highly porous 3-dimensional polyphosphazene polymer matrix for skeletal tissue regeneration", J. Biomed. Mater. Res. 1996 30:133-138. Lewis et al., "Use of Microgravity Bioreactors for Development of an In Vitro Rat Salivary Gland Cell Culture Model", J. Cellular Biochem. 1993 51:265-273. Masi et al., "Adhesion, Growth, and Matrix Production by Osteoblasts on Collagen Substrata", Calcified Tissue International 1992 51:202-212. Mizuno et al., Osteogenesis by Bone Marrow Stromal Cells Maintained on Type I Collagen Matrix Gels In Vivo, Bone 1997 20(2):101107.